The Secret Behind the World’s Oldest Living Trio of Sisters
Three Brazilian sisters have been recognized by Guinness World Records as the oldest living trio of siblings in the world, with a combined age of 316 years. Levita de Deus Nunes (109), Zoraide de Deus Mota (104), and Zulina de Deus Nunes (103) are now the subjects of the “DNA Longevo” study, a research project led by geneticist Mayana Zatz at the Sao Paulo University, which seeks to identify the biological factors behind extreme longevity.
Did You Know?
The three sisters, who currently reside in Rio de Janeiro, credit their longevity to a lifetime of active living and a diet of fresh foods. Zulina de Deus Nunes recalls a childhood spent swimming and fishing in rivers where they did not have a refrigerator.
The Scientific Search for Genetic Markers
Researchers are analyzing the DNA of centenarians to isolate “protective genes” that may shield vital organs from the typical effects of aging. According to Mayana Zatz, who coordinates the Human Genome Research Center, the goal is to compare genetic data from healthy individuals in their 90s and 100s against those suffering from chronic illness, physical weakness, or cognitive decline. Scientists believe that genetics play a more significant role in maintaining health at an advanced age than environmental factors.
Expert Insight:
While the genetic component of extreme longevity is significant, Ben Meyers, CEO of LongeviQuest—the organization that verified the sisters’ record—notes that because they live close to each other, they have a support network, with family who can help if needed. He adds that there is a community aspect as well.
Future Implications for Longevity Research
The “DNA Longevo” project aims to build a database of 500 individuals aged 100 or older to establish more accurate medical conclusions regarding human aging. Researcher Joao Paulo Guilherme states that mapping how genetics protect the heart, muscles, and brain could eventually lead to new medical insights.

Frequently Asked Questions
Who are the three oldest living siblings?
The siblings are Levita de Deus Nunes (109), Zoraide de Deus Mota (104), and Zulina de Deus Nunes (103), all residing in Brazil.
What is the purpose of the DNA Longevo study?
Led by Mayana Zatz, the study aims to identify protective genes by comparing the DNA of healthy centenarians with individuals who experience cognitive or physical decline.
What factors do the sisters attribute to their long lives?
The sisters point to a combination of an active lifestyle, a diet consisting of fresh foods, breastfeeding, and the importance of having a supportive family network.
What environmental or lifestyle changes do you think are most important for maintaining health in later years?